Siena — Siena, 184 км |
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| 1 | Julian Alaphilippe | (Fra) | Deceuninck — Quick Step | 4:47:14 |
| 2 | Jakob Fuglsang | (Den) | Astana Pro Team | 0:00:02 |
| 3 | Wout Van Aert | (Bel) | Team Jumbo — Visma | 0:00:27 |
| 4 | Zdenek Štybar | (Cze) | Deceuninck — Quick Step | 0:01:00 |
| 5 | Tiesj Benoot | (Bel) | Lotto Soudal | |
| 6 | Greg Van Avermaet | (Bel) | CCC Team | 0:01:01 |
| 7 | Alexey Lutsenko | (Kaz) | Astana Pro Team | 0:01:04 |
| 8 | Simon Clarke | (Aus) | EF Education First | 0:01:08 |
| 9 | Toms Skujins | (Lat) | Trek — Segafredo | 0:01:12 |
| 10 | Tim Wellens | (Bel) | Lotto Soudal | 0:01:21 |
| 11 | Yves Lampaert | (Bel) | Deceuninck — Quick Step | 0:01:28 |
| 12 | Geraint Thomas | (Gbr) | Team Sky | 0:02:41 |
| 13 | Pieter Serry | (Bel) | Deceuninck — Quick Step | |
| 14 | Silvan Dillier | (Sui) | AG2R La Mondiale | 0:02:42 |
| 15 | Stefan Küng | (Sui) | Groupama — FDJ | 0:02:44 |
| 16 | Marco Canola | (Ita) | Nippo — Vini Fantini — Faizanè | |
| 17 | Sam Oomen | (Ned) | Team Sunweb | |
| 18 | Luke Durbridge | (Aus) | Mitchelton — Scott | 0:02:47 |
| 19 | Christopher Juul-Jensen | (Den) | Mitchelton — Scott | 0:02:48 |
| 20 | Roman Kreuziger | (Cze) | Team Dimension Data | |
| 21 | Brent Bookwalter | (Usa) | Mitchelton — Scott | |
| 22 | Ruben Guerreiro | (Por) | Team Katusha Alpecin | 0:02:56 |
| 23 | Davide Ballerini | (Ita) | Astana Pro Team | 0:02:57 |
| 24 | Diego Rosa | (Ita) | Team Sky | 0:03:00 |
| 25 | Nelson Oliveira | (Por) | Movistar Team | 0:03:06 |
| 26 | Viacheslav Kuznetsov | (Rus) | Team Katusha Alpecin | 0:03:09 |
| 27 | Romain Seigle | (Fra) | Groupama — FDJ | 0:03:17 |
| 28 | Salvatore Puccio | (Ita) | Team Sky | 0:06:54 |
| 29 | Maximilian Schachmann | (Ger) | Bora — Hansgrohe | |
| 30 | Tadej Pogacar | (Slo) | UAE Team Emirates | 0:08:04 |
| 31 | Vincenzo Nibali | (Ita) | Bahrain Merida | 0:08:57 |
| 32 | Rob Power | (Aus) | Team Sunweb | |
| 33 | Nathan Van Hooydonck | (Bel) | CCC Team | 0:09:17 |
| 34 | Michael Schär | (Sui) | CCC Team | |
| 35 | Quentin Jauregui | (Fra) | AG2R La Mondiale | |
| 36 | Benjamin King | (Usa) | Team Dimension Data | |
| 37 | Jan Tratnik | (Slo) | Bahrain Merida | |
| 38 | Sebastian Schönberger | (Aut) | Neri Sottoli — Selle Italia — KTM | |
| 39 | Luka Pibernik | (Slo) | Bahrain Merida | |
| 40 | Hermann Pernsteiner | (Aut) | Bahrain Merida | |
| 41 | Simone Velasco | (Ita) | Neri Sottoli — Selle Italia — KTM | |
| 42 | Floris De Tier | (Bel) | Team Jumbo — Visma | |
| 43 | Rafal Majka | (Pol) | Bora — Hansgrohe | |
| 44 | Simone Petilli | (Ita) | UAE Team Emirates | |
| 45 | Michael Valgren Andersen | (Den) | Team Dimension Data | 0:09:24 |
| 46 | Dries Devenyns | (Bel) | Deceuninck — Quick Step | |
| 47 | Enrico Battaglin | (Ita) | Team Katusha Alpecin | |
| 48 | Jai Hindley | (Aus) | Team Sunweb | |
| 49 | Robert Stannard | (Aus) | Mitchelton — Scott | |
| 50 | Jens Keukeleire | (Bel) | Lotto Soudal | 0:09:26 |
| 51 | Dario Cataldo | (Ita) | Astana Pro Team | |
| 52 | Jimmy Turgis | (Fra) | Vital Concept — B&B Hotels | 0:09:30 |
| 53 | Lukasz Wisniowski | (Pol) | CCC Team | |
| 54 | Michal Golas | (Pol) | Team Sky | |
| 55 | Cesare Benedetti | (Ita) | Bora — Hansgrohe | |
| 56 | Eduardo Sepulveda | (Arg) | Movistar Team | 0:09:44 |
| 57 | Antoine Duchesne | (Can) | Groupama — FDJ | 0:09:46 |
| 58 | Gianni Moscon | (Ita) | Team Sky | 0:09:57 |
| 59 | François Bidard | (Fra) | AG2R La Mondiale | |
| 60 | Petr Vakoc | (Cze) | Deceuninck — Quick Step | 0:12:05 |
| 61 | Jonathan Caicedo | (Ecu) | EF Education First | 0:12:24 |
| 62 | Richard Carapaz | (Ecu) | Movistar Team | |
| 63 | Guillaume Van Keirsbulck | (Bel) | CCC Team | 0:12:27 |
| 64 | Eduard Prades Reverter | (Esp) | Movistar Team | |
| 65 | Owain Doull | (Gbr) | Team Sky | |
| 66 | Ben Gastauer | (Lux) | AG2R La Mondiale | |
| 67 | Taco Van Der Hoorn | (Ned) | Team Jumbo — Visma | |
| 68 | Reinardt Janse Van Rensburg | (Rsa) | Team Dimension Data | |
| 69 | Gijs Van Hoecke | (Bel) | CCC Team | |
| 70 | Tomasz Marczynski | (Pol) | Lotto Soudal | |
| 71 | Dmitrii Strakhov | (Rus) | Team Katusha Alpecin | 0:12:32 |
| 72 | Tanel Kangert | (Est) | EF Education First | |
| 73 | Marc Hirschi | (Sui) | Team Sunweb | |
| 74 | Oscar Gatto | (Ita) | Bora — Hansgrohe | 0:12:43 |
| 75 | Rasmus Fossum Tiller | (Nor) | Team Dimension Data | 0:12:47 |
| 76 | Bauke Mollema | (Ned) | Trek — Segafredo | 0:12:49 |
| 77 | Nico Denz | (Ger) | AG2R La Mondiale | 0:12:57 |
| 78 | Alberto Bettiol | (Ita) | EF Education First | 0:22:54 |
| 79 | Nathan Haas | (Aus) | Team Katusha Alpecin | |
| 80 | Damiano Cima | (Ita) | Nippo — Vini Fantini — Faizanè | |
| 81 | Nicholas Schultz | (Aus) | Mitchelton — Scott | |
| 82 | Andrea Garosio | (Ita) | Bahrain Merida | |
| 83 | Carlos Betancur | (Col) | Movistar Team | 0:22:57 |
| OTL | Christopher Hamilton | (Aus) | Team Sunweb | |
| OTL | Vegard Stake Laengen | (Nor) | UAE Team Emirates | |
| OTL | Lawrence Naesen | (Bel) | Lotto Soudal | |
| OTL | Markel Irizar Aramburu | (Esp) | Trek — Segafredo | |
| OTL | Zhandos Bizhigitov | (Kaz) | Astana Pro Team | |
| OTL | Jaco Venter | (Rsa) | Team Dimension Data | |
| OTL | Lennard Hofstede | (Ned) | Team Jumbo — Visma | |
| OTL | Nicolas Roche | (Irl) | Team Sunweb | |
| OTL | Edoardo Affini | (Ita) | Mitchelton — Scott | |
| OTL | Fumiyuki Beppu | (Jpn) | Trek — Segafredo | |
| OTL | Logan Owen | (Usa) | EF Education First | |
| OTL | Eros Capecchi | (Ita) | Deceuninck — Quick Step | |
| OTL | Nicola Conci | (Ita) | Trek — Segafredo | |
| OTL | Giovanni Lonardi | (Ita) | Nippo — Vini Fantini — Faizanè | |
| OTL | Grega Bole | (Slo) | Bahrain Merida | |
| OTL | Luca Raggio | (Ita) | Neri Sottoli — Selle Italia — KTM | |
| OTL | Dmitriy Gruzdev | (Kaz) | Astana Pro Team | |
| OTL | Lachlan Morton | (Aus) | EF Education First | |
| DNF | Carl Fredrik Hagen | (Nor) | Lotto Soudal | |
| DNF | Tosh Van Der Sande | (Bel) | Lotto Soudal | |
| DNF | Alexandre Geniez | (Fra) | AG2R La Mondiale | |
| DNF | Nans Peters | (Fra) | AG2R La Mondiale | |
| DNF | Manuele Boaro | (Ita) | Astana Pro Team | |
| DNF | Antonio Nibali | (Ita) | Bahrain Merida | |
| DNF | Maciej Bodnar | (Pol) | Bora — Hansgrohe | |
| DNF | Gregor Mühlberger | (Aut) | Bora — Hansgrohe | |
| DNF | Daniel Oss | (Ita) | Bora — Hansgrohe | |
| DNF | Josef Cerný | (Cze) | CCC Team | |
| DNF | Sean Bennett | (Usa) | EF Education First | |
| DNF | Bruno Armirail | (Fra) | Groupama — FDJ | |
| DNF | Tobias Ludvigsson | (Swe) | Groupama — FDJ | |
| DNF | Matthieu Ladagnous | (Fra) | Groupama — FDJ | |
| DNF | Léo Vincent | (Fra) | Groupama — FDJ | |
| DNF | Callum Scotson | (Aus) | Mitchelton — Scott | |
| DNF | Carlos Barbero | (Esp) | Movistar Team | |
| DNF | José Rojas | (Esp) | Movistar Team | |
| DNF | Giovanni Visconti | (Ita) | Neri Sottoli — Selle Italia — KTM | |
| DNF | Liam Bertazzo | (Ita) | Neri Sottoli — Selle Italia — KTM | |
| DNF | Luca Pacioni | (Ita) | Neri Sottoli — Selle Italia — KTM | |
| DNF | Giuseppe Fonzi | (Ita) | Neri Sottoli — Selle Italia — KTM | |
| DNF | Moreno Moser | (Ita) | Nippo — Vini Fantini — Faizanè | |
| DNF | Nicola Bagioli | (Ita) | Nippo — Vini Fantini — Faizanè | |
| DNF | Sho Hatsuyama | (Jpn) | Nippo — Vini Fantini — Faizanè | |
| DNF | Juan José Lobato Del Valle | (Esp) | Nippo — Vini Fantini — Faizanè | |
| DNF | Gino Mäder | (Sui) | Team Dimension Data | |
| DNF | Neilson Powless | (Usa) | Team Jumbo — Visma | |
| DNF | Antwan Tolhoek | (Ned) | Team Jumbo — Visma | |
| DNF | Danny Van Poppel | (Ned) | Team Jumbo — Visma | |
| DNF | Steff Cras | (Bel) | Team Katusha Alpecin | |
| DNF | Ian Boswell | (Usa) | Team Katusha Alpecin | |
| DNF | Leonardo Basso | (Ita) | Team Sky | |
| DNF | Asbjørn Kragh Andersen | (Den) | Team Sunweb | |
| DNF | Fabio Felline | (Ita) | Trek — Segafredo | |
| DNF | Matteo Moschetti | (Ita) | Trek — Segafredo | |
| DNF | Fernando Gaviria Rendon | (Col) | UAE Team Emirates | |
| DNF | Rui Costa | (Por) | UAE Team Emirates | |
| DNF | Roberto Ferrari | (Ita) | UAE Team Emirates | |
| DNF | Jasper Philipsen | (Bel) | UAE Team Emirates | |
| DNF | Yoann Bagot | (Fra) | Vital Concept — B&B Hotels | |
| DNF | Maxime Cam | (Fra) | Vital Concept — B&B Hotels | |
| DNF | Bert De Backer | (Bel) | Vital Concept — B&B Hotels | |
| DNF | Corentin Ermenault | (Fra) | Vital Concept — B&B Hotels | |
| DNF | Steven Lammertink | (Ned) | Vital Concept — B&B Hotels | |
| DNF | Justin Mottier | (Fra) | Vital Concept — B&B Hotels | |
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